pdgf-bb, human  (MedChemExpress)

Journal: Science Advances

Article Title: Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1B

doi: 10.1126/sciadv.adv5362

Figure Lengend Snippet: ( A ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min, and subsequently treated with 10 μM PDGFR inhibitor (PDGFRi) for 5, 10, and 20 min. Cells were fixed, permeabilized at the indicated times, and subsequently evaluated with primary antibodies from different species for hPTP1B (mouse) and GLRX1 (goat) to reveal protein-protein interactions in situ using PLA (red spots). Nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI; blue). ( B ) Visualization and quantification were performed using fluorescent microscopy and ImageJ. Statistical differences in signal quantification are indicated in the bar graph (* P < 0.05; ** P < 0.01); n ≥ 3. Error bars denote SEM. ( C ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min or with 100 μM H 2 O 2 for 10 min, with and without catalase pretreatment (2 hours; 50 U/ml) as indicated. The images display results from one representative experiment, out of three independent experiments. Scale bars, 30 μm. Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc tests for multiple comparisons with GraphPad Prism.

Article Snippet: After overnight starvation, the cells were stimulated with either EGF or PDGF (R&D Systems, catalog no. 220-BB) ligand for 4 min.

Techniques: Protein-Protein interactions, In Situ, Microscopy

Journal: Wound Repair and Regeneration

Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes

doi: 10.1111/wrr.70153

Figure Lengend Snippet: Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification of PDGF‐BB (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.

Article Snippet: The concentrations of platelet‐derived growth factor‐BB (PDGF‐BB) (E‐EL‐H1577, Elabscience, USA), P‐selectin (SEA569Hu; Cloud‐Clone Corp, USA) and CCL‐5 (Proteintech, USA) were determined using sandwich ELISA assays and the absorbance read at 450 nm using a microplate reader (Multiskan GO 1.00.40; Thermo‐Scientific Group, USA) according to the manufacturer's instructions.

Techniques: Comparison, Control